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| Registros recuperados: 14 | |
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| Bes, Meline; Merrouch, Meriem; Joseph, Manon; Quemeneur, Marianne; Payri, Claude; Pelletier, Bernard; Ollivier, Bernard; Fardeau, Marie-laure; Erausol, Gael; Postec, Anne. |
| A novel anaerobic bacterial strain, ST07-YET, was isolated from a carbonate chimney of the Prony Hydrothermal Field (PHF) in New Caledonia. Cells were Gram-stain-positive, straight rods (0.7-0.8 x 3.0-5.0 mu m) and motile by means of lateral flagella. Strain ST07-YET was mesophilic (optimum 35 degrees C), moderately alkaliphilic and halotolerant (optimum pH 8.7 and 5 gr l(-1) NaCl). Elemental sulfur, sulfate, thiosulfate, sulfite, nitrate and nitrite were not used as terminal electron acceptors. Yeast extract, peptone, tryptone, Casamino acids, crotonate, pyruvate, galactose, maltose, sucrose, ribose, trehalose and glucose were used as carbon sources. Glucose fermentation led to acetate, H-2 and CO2 formation. Arginine, serine, histidine, lysine,... |
| Tipo: Text |
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| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00467/57917/60305.pdf |
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| Postec, Anne; Urios, Laurent; Lesongeur, Francoise; Ollivier, Bernard; Querellou, Joel; Godfroy, Anne. |
| The microflora developing during a continuous enrichment culture from a hydrothermal chimney sample was investigated by molecular methods. The culture was performed in a gas-lift bioreactor under anaerobic conditions, at 90 degrees C and pH 6.5, on a complex medium containing sulfur as the terminal electron acceptor. Archaeal and bacterial diversity was studied. Microorganisms affiliated with the genera Pyrococcus, Marinitoga, and Bacillus were detected through DGGE analysis of 16S rDNA. Additional sequences phylogenetically related to Thermococus and epsilon-Proteobacteria were detected by cloning and sequencing of 16S rDNA from two samples of the enrichment culture. In comparison, the sequences retrieved from cloning analysis from an enrichment culture... |
| Tipo: Text |
Palavras-chave: Sequence alignment; Anaerobic digester; Thermotogales; Thermophile; Deep sea vent. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-364.pdf |
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| Postec, Anne; Lesongeur, Francoise; Pignet, Patricia; Ollivier, B; Querellou, Joel; Godfroy, Anne. |
| The prokaryotic diversity of culturable thermophilic communities of deep-sea hydrothermal chimneys was analysed using a continuous enrichment culture performed in a gas-lift bioreactor, and compared to classical batch enrichment cultures in vials. Cultures were conducted at 60 degrees C and pH 6.5 using a complex medium containing carbohydrates, peptides and sulphur, and inoculated with a sample of a hydrothermal black chimney collected at the Rainbow field, Mid-Atlantic Ridge, at 2,275 m depth. To assess the relevance of both culture methods, bacterial and archaeal diversity was studied using cloning and sequencing, DGGE, and whole-cell hybridisation of 16S rRNA genes. Sequences of heterotrophic microorganisms belonging to the genera Marinitoga,... |
| Tipo: Text |
Palavras-chave: Metabolic interactions; 16S rRNA gene; Thermophiles; Bioreactor; Continuous enrichment cultures; Deep sea hydrothermal vent; Microbial diversity. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3885.pdf |
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| Lakhal, Raja; Pradel, Nathalie; Postec, Anne; Ivier, Bernard; Cayol, Jean-luc; Godfroy, Anne; Fardeau, Marie-laure; Gales, Gregoire. |
| A novel, anaerobic, chemo-organotrophic bacterium, designated strain Ra1766HT, was isolated from sediments of the Guaymas basin (Gulf of California, Mexico) taken from a depth of 2002 m. Cells were thin, motile, Gram-stain-positive, flexible rods forming terminal endospores. Strain Ra1766H(T) grew at temperatures of 25-45 degrees C (optimum 30 degrees C), pH 6.7-8.1 (optimum 7.5) and in a salinity of 5-60 g l(-1) NaCl (optimum 30 g l(-1)). It was an obligate heterotrophic bacterium fermenting carbohydrates (glucose and mannose) and organic acids (pyruvate and succinate). Casamino acids and amino acids (glutamate, aspartate and glycine) were also fermented. The main end products from glucose fermentation were acetate, butyrate, ethanol, H-2 and CO2.... |
| Tipo: Text |
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| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00296/40742/39992.pdf |
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| Postec, Anne; Le Breton, Claire; Fardeau, Marie-laure; Lesongeur, Francoise; Pignet, Patricia; Querellou, Joel; Ollivier, Bernard; Godfroy, Anne. |
| A novel, thermophilic, anaerobic bacterium that is able to tolerate hydrogen was isolated from a deep-sea hydrothermal chimney collected at the Rainbow field on the Mid-Atlantic Ridge. Cells were rod-shaped and surrounded by a sheath-like outer structure (toga); they were weakly motile by means of a polar flagellum. They appeared singly, in pairs or in short chains. They grew at 35-65 degrees C (optimum 60 degrees C), pH 4.5-8.5 (optimum pH 6.0) and 10-65 g sea salts l(-1) (optimum 30-40 g l(-1)). The isolate was organotrophic, and able to grow on various carbohydrates or complex proteinaceous substrates. Growth was not inhibited under 100% hydrogen or in the presence of 2% oxygen in the gas phase. The isolate reduces sulfur, although sulfur reduction is... |
| Tipo: Text |
Palavras-chave: Anaerobic bacterium; Marinitoga hydrogenitolerans; Thermotogales; Thermophile; Deep sea vent. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-363.pdf |
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| Mei, Nan; Postec, Anne; Monnin, Christophe; Pelletier, Bernard; Payri, Claude E.; Menez, Benedicte; Frouin, Eleonore; Ollivier, Bernard; Erauso, Gael; Quemeneur, Marianne. |
| High amounts of hydrogen are emitted in the serpentinite-hosted hydrothermal field of the Prony Bay (PHF, New Caledonia), where high-pH (similar to 11), low-temperature (< 40 degrees C), and low-salinity fluids are discharged in both intertidal and shallow submarine environments. In this study, we investigated the diversity and distribution of potentially hydrogen-producing bacteria in Prony hyperalkaline springs by using metagenomic analyses and different PCR-amplified DNA sequencing methods. The retrieved sequences of hydA genes, encoding the catalytic subunit of [FeFe]-hydrogenases and, used as a molecular marker of hydrogen-producing bacteria, were mainly related to those of Firmicutes and clustered into two distinct groups depending on sampling... |
| Tipo: Text |
Palavras-chave: Hydrogen; Microbial diversity; Hydrogen producers; Serpentinization; HydA genes; [FeFe]-hydrogenase; Metagenomics. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00467/57913/60308.pdf |
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| Postec, Anne; Quemeneur, Marianne; Bes, Meline; Mei, Nan; Benaissa, Fatma; Payri, Claude; Pelletier, Bernard; Monnin, Christophe; Guentas-dombrowsky, Linda; Ollivier, Bernard; Gerard, Emmanuelle; Pisapia, Celine; Gerard, Martine; Menez, Benedicte; Erauso, Gael. |
| Active carbonate chimneys from the shallow marine serpentinizing Prony Hydrothermal Field were sampled 3 times over a 6 years period at site ST09. Archaeal and bacterial communities composition was investigated using PCR-based methods (clone libraries, Denaturating Gel Gradient Electrophoresis, quantitative PCR) targeting 16S rRNA genes, methyl coenzyme M reductase A and dissimilatory sulfite reductase subunit B genes. Methanosarcinales (Euryarchaeota) and Thaumarchaea were the main archaeal members. The Methanosarcinales, also observed by epifluorescent microscopy and FISH, consisted of two phylotypes that were previously solely detected in two other serpentinitzing ecosystems (The Cedars and Lost City Hydrothermal Field). Surprisingly, members of the... |
| Tipo: Text |
Palavras-chave: Hydrothermal; Serpentinization; Alkaline; Prony; Microbial communities. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00467/57916/60307.pdf |
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| Pisapia, Celine; Gerard, Emmanuelle; Gerard, Martine; Lecourt, Lena; Lang, Susan Q.; Pelletier, Bernard; Payri, Claude E.; Monnin, Christophe; Guentas, Linda; Postec, Anne; Quemeneur, Marianne; Erauso, Gael; Menez, Benedicte. |
| Despite their potential importance as analogs of primitive microbial metabolisms, the knowledge of the structure and functioning of the deep ecosystems associated with serpentinizing environments is hampered by the lack of accessibility to relevant systems. These hyperalkaline environments are depleted in dissolved inorganic carbon (DIC), making the carbon sources and assimilation pathways in the associated ecosystems highly enigmatic. The Prony Bay Hydrothermal Field (PHF) is an active serpentinization site where, similar to Lost City (Mid-Atlantic Ridge), high-pH fluids rich in H-2 and CH4 are discharged from carbonate chimneys at the seafloor, but in a shallower lagoonal environment. This study aimed to characterize the subsurface microbial ecology of... |
| Tipo: Text |
Palavras-chave: Serpentinization; Alkaline hydrothermalism; Deep life; Firmicutes; Acetothermia; Omnitrophica; Organic carbon. |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00467/57909/60314.pdf |
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| Postec, Anne; Pignet, Patricia; Cueff-gauchard, Valerie; Schmitt, Anne; Querellou, Joel; Godfroy, Anne. |
| The hyperthermophilic archaeon Thermococcus hydrothermalis was cultivated in continuous culture in a gas-lift bioreactor in the absence of elemental sulphur on both proteinaceous and maltose-containing media. Optimal conditions (pH, temperature and gas flow rate), determined on complex media that yielded maximal growth rate and maximal steady state cell density, were obtained at 80degreesC, pH 6 and gas sparging at 0.2 v v(-1) min(-1). Higher steady state cell densities were obtained on a medium containing maltose and yeast extract. In order to design a defined and minimal media, the nutritional requirements of T hydrothermalis were then investigated using continuous culture in the absence of elemental sulphur in the gas-lift bioreactor. First, the complex... |
| Tipo: Text |
Palavras-chave: Bioreactor; Defined and minimal media; Culture optimisation; Gas lift bioreactor; Thermococcus hydrothermalis. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-365.pdf |
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| Mei, Nan; Postec, Anne; Erauso, Gael; Joseph, Manon; Pelletier, Bernard; Payri, Claude; Ollivier, Bernard; Quemeneur, Marianne. |
| A novel anaerobic, alkaliphilic, Gram-stain-positive, spore-forming bacterium was isolated from a carbonaceous hydrothermal chimney in Prony Bay, New Caledonia. This bacterium, designated strain 3b(T), grew at temperatures from 30 to 43 degrees C (optimum 37 degrees C) and at pH between 7.8 and 10.1 (optimum 9.5). Added NaCl was not required for growth (optimum 0-0.2 %, w/v), but was tolerated at up to 4 %. Yeast extract was required for growth. Strain 3b(T) utilized crotonate, lactate and pyruvate, but not sugars. Crotonate was dismutated to acetate and butyrate. Lactate was disproportionated to acetate and propionate. Pyruvate was degraded to acetate plus trace amounts of hydrogen. Growth on lactate was improved by the addition of fumarate, which was... |
| Tipo: Text |
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| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00467/57912/60312.pdf |
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| Urios, Laurent; Cueff-gauchard, Valerie; Pignet, Patricia; Postec, Anne; Fardeau, Marie-laure; Ollivier, Bernard; Barbier, Georges. |
| A novel anaerobic, thermophilic and heterotrophic bacterium, designated strain DV114O(T), was isolated from a deep-sea hydrothermal vent sample from the Mid-Atlantic Ridge. The cells were non-motile straight rods, 1-8 mum long and 0-4 mum wide, surrounded by an outer sheath-like structure (toga). They grew at 45-80 degreesC (optimum 65 degreesC), pH 5-0-9-0 (optimum pH 6-0) and at sea salt concentrations of 20-60 gl(-1) (optimum 30 gl(-1)). Strain DV1140(T) was able to ferment yeast extract, peptone, brain heart infusion, gelatin, starch, galactose, arabinose, glucose, trehalose and cellobiose. The fermentation products identified on glucose in the presence of yeast extract and peptone were acetate, isovalerate and hydrogen. The G + C content of the... |
| Tipo: Text |
Palavras-chave: Marine microorganisms; Thermosipho atlanticus; Thermotogales; Thermophile; Deep sea vent. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-362.pdf |
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| Lakhal, Raja; Pradel, Nathalie; Postec, Anne; Hamdi, Moktar; Ollivier, Bernard; Godfroy, Anne; Fardeaul, Marie-laure. |
| A novel obligately anaerobic, non-spore-forming, rod-shaped mesophilic, halophilic, Gram-stain-negative bacterium, was isolated from sediments of Guaymas Basin. The strain, designated Ra1766G1(T), grew at 20-40 degrees C (optimum, 30-35 degrees C) and at pH 6.0-8.0 (optimum, pH 6.5-7.5). It required 0.5-7.5 % NaCl (optimum, 2-3 %) for growth. Sulfate, thiosulfate, elemental sulfur, sulfite, fumarate, nitrate and nitrite were not used as terminal electron acceptors. Strain Ra1766G1(T) used cellobiose, glucose, mannose, maltose, arabinose, raffinose, galactose, ribose, sucrose, pyruvate and xylose as electron donors. The main fermentation product from glucose metabolism was acetate. The predominant cellular fatty acids were anteiso-C-15:0, iso-C-15:0,... |
| Tipo: Text |
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| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00177/28821/27463.pdf |
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| Registros recuperados: 14 | |
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